David A. Rubenstein
My lab has two major focuses; the first being vascular tissue engineering and the second being the initiation/progression of cardiovascular diseases mediated through platelet and endothelial cell interactions. For our tissue engineering projects, we design and fabricate novel three dimensional biomimetic scaffolds using electrospinning. These scaffolds can be fabricated with tailored mechanical, chemical and topographical properties to match the native vascular extracellular matrix properties. After successful fabrication, we test the scaffolds for mechanical properties and physical properties, using various techniques, such as nanoindentation, goniometry and Raman spectroscopy. Ideal scaffolds are then tested for biocompatibility towards endothelial cells and platelets using cell culture methods that have been developed by us. We specifically are interested in whether or not pro-angiogenic properties are enhanced and pro-inflammatory/pro-thrombotic properties are inhibited. We use both two-dimensional and three-dimensional culture techniques. Finally, our most promising scaffolds can be implanted in mouse models todetermine whether or not wound healing is enhanced throughout these scaffolds. Our long-term goal is to design a scaffold that promotes and/or accelerates wound healing in various applications.
Our second focus involves experiments that are designed to determine the mechanism(s) that instigate cardiovascular disease progression. Specifically, we are interested in how tobacco smoke products, hyperlipidemia, advanced glycation end products and disturbed shear stress can alter the progression of cardiovascular diseases. We currently, use various in vitro techniques to investigate platelet and endothelial cell responses to these various cardiovascular disease risk factors. Additionally, we are looking into various mouse models of these diseases to use a more physiologically relevant setting for our investigations. Our long-term goal is to understand disease progression and identify new biomarkers and/or therapeutic targets for disease intervention. Recently, we have become interested in how the liver and specifically the actions of Kupffer cells can instigate cardiovascular diseases.
- Ph.D. - Biomedical Engineering, Stony Brook University, 2007.
- M.S. - Biomedical Engineering, Stony Brook University, 2005.
- B.E. - Biomedical Engineering, Stony Brook University, 2004.
- 2015 - Associate Professor, Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York
- 2013 - 2015 Assistant Professor, Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York.
- 2007 - 2013 Assistant Professor, School of Mechanical and Aerospace Engineering, Oklahoma State University, Stillwater, Oklahoma.
- Halliburton Excellent Young Teacher (2012)
- Mechanical and Aerospace Engineering – Golden Screw Award (AY 09-10)
- Mechanical and Aerospace Engineering Outstanding Faculty Member in Teaching and Research (AY 08-09)
- Award for Academic Excellence in Biomedical Engineering – Class of 2004)
- Provosts Award for Academic Excellence – State University of New York at Stony Brook (May 2004)
- Barry M. Goldwater Scholar (2003)
- National Science Foundation – BMAT Polymers, Gel and Matrices Panel 2
- American Heart Association – Vascular Wall Biology Panel (2008-present)
- National Institutes of Health – Biomaterials and Biointerfaces Study Section
- Journals: Microcirculation, Annals of Biomedical Engineering, Journal of Thrombosis and Haemostasis, Thrombosis Research, Journal of Diabetes and Metabolism, American Journal of Physiology: Heart and Circulatory Physiology, Acta Biomaterialia, Tissue Engineering, Journal of Physiology and Biochemistry, Biophysical Journal, Journal of Biomedical Materials Research: Part A, among others
Membership in Professional Societies and Committees:
- Member, Biomedical Engineering Society (Awards Committee)
- Member, Microcirculatory Society (Awards/Communications Committee, Chair of Communications Committee)
- Member, American Physiological Society
Click here to search David Rubenstein's PubMed listings
Books/Contribution to Books
- Wei Yin and David A. Rubenstein. Chapter 30: Biomedical Applications of Aerogels in Aerogels Handbook edited by Michael Aegerter, Nicholas Leventis and Matthias Koebel. Springer; New York, 2011. ISBN-13: 978-1-4419-7477-8.
- David A. Rubenstein, Wei Yin and Mary D. Frame. Biofluid Mechanics: An Introduction to Biofluid Mechanics, Macrocirculation and Microcirculation. Elsevier; Massachusetts, 2012. ISBN-13: 978-0-12-381383-1.
- David A. Rubenstein, Wei Yin and Mary D. Frame. Biofluid Mechanics: An Introduction to Biofluid Mechanics, Macrocirculation and Microcirculation. 2nd Edition. Elsevier; Massachusetts, 2015. ISBN-13: 978-0-12-800944-4.
Selected Peer Reviewed Journal Papers
- Characterization of the biocompatibility and mechanical properties of polyurea organic aerogels with blood and vascular endothelial cells -- aerogel potential applications in blood implantable devices. Wei Yin, Hongbing Lu, Nicholas Leventis and David A. Rubenstein. International Journal of Polymeric Materials and Polymeric Biomaterials, 62:2, 109-118, 2013 (DOI: 10.1080/00914037.2012.698339).
- Differences between mainstream and sidestream tobacco smoke extracts and nicotine in the activation and aggregation of platelets subjected to cardiovascular conditions in diabetes. Wei Yin and David A. Rubenstein. Diabetes and Vascular Disease Research. Volume 10, Issue 1, Pages 57-64, 2013 (DOI: 10.1177/1479164112445282).
- A Biocompatible Flow Chamber to Study the Hemodynamic Performance of Prosthetic Heart Valves. Wei Yin, Ek Ching Ngwe and David A. Rubenstein. ASAIO Journal. Volume 58, Issue 5, Pages: 470-480, September/October 2012 (DOI: 10.1097/MAT.0b013e31826140a2).
- Glycated albumin and pathological shear stress alters endothelial cell thrombogenic potential, pro-inflammatory state and cytoskeletal dynamics. Zahra Maria, Wei Yin and David A. Rubenstein. Journal of Diabetes and Metabolism, 2011 S4-003 (DOI: 10.4172/2155-6156.S4-003).
- Characterization of the Physical Properties and Biocompatibility of Polybenzoxazine Based Aerogels for use as a Novel Hard Tissue Scaffold. David A. Rubenstein, Hongbing Lu, Shruti S. Mahadik, Nicholas Leventis and Wei Yin. Journal of Biomaterials Science: Polymer Edition, Volume 23, Issue 9: Pages 1171-1184, 2012 (DOI: 10.1163/092050611X576954).
- Effects of physiologically relevant dynamic shear stress on platelet complement activation. Saravan Kumar Shanmugavelayudam, David A. Rubenstein and Wei Yin. Platelets, December 2011; 22(8): 602-610 (DOI: 10.3109/09537104.2011.585257).
- Endothelial Cell Adhesion and Growth within a Bioassay Chamber using Microstamped ECM Proteins. David A. Rubenstein and Mary D. Frame. JOM, June 2011; Volume 63, Issue 6: 84-93 (DOI: 10.1007/s11837-011-0097-z).
- Glycated albumin modulates endothelial cell thrombogenic and inflammatory responses. David A. Rubenstein, Zahra Maria and Wei Yin. Journal of Diabetes Science and Technology, May 2011; Volume 5, Issue 3: 703-713.
- The effect of physiologically relevant dynamic shear stress on platelet and endothelial cell activation. Wei Yin, Saravan Kumar Shanmugavelayudam and David A. Rubenstein. Thrombosis Research, 2011; Volume 127: 235-241 (DOI: 10.1016/j.thromres.2010.11.021).
- In Vitro Biocompatibility of Sheath-Core Cellulose-Acetate-Based Electrospun Scaffolds Towards Endothelial Cells and Platelets. David A. Rubenstein, Subramaniam M. Venkitachalam, Dan Zamfir, Fang Wang, Hongbing Lu, Mary D. Frame and Wei Yin. Journal of Biomaterials Science: Polymer Edition, 2010; Volume 21: 1713-1736. (DOI 10.1163/092050609X12559317149363).
- The combined effects of sidestream smoke extracts and glycated serum albumin on endothelial cells and platelets. David A. Rubenstein, Blake E. Morton and Wei Yin. Cardiovascular Diabetology 2010, 9:28. (DOI:10.1186/1475-2840-9-28).
- Quantifying the Effects of Shear Stress and Shear Exposure Duration Regulation on Flow Induced Platelet Activation and Aggregation. David A. Rubenstein and Wei Yin. Journal of Thrombosis and Thrombolysis: Volume 30, Issue 1 (2010): 36-45. (DOI: 10.1007/s11239-009-0397-0).
- Effect of geometrical assumptions on numerical modeling of coronary blood flow under normal and disease conditions. Saravan Kumar Shanmugavelayudam, David A. Rubenstein and Wei Yin. Journal of Biomechanical Engineering. 2010, June, Vol. 132, Issue. 6, 061004-1 to 061004-8. (DOI: 10.1115/1.4001033).
- Biocompatibility of Surfactant-Templated Polyurea Nanoencapsulated Macroporous Templated Silica Aerogels with Plasma, Platelets and Endothelial Cells. Wei Yin, Subramaniam M. Venkitachalam, Ellen Jarrett, Sarah Staggs, Nicholas Leventis, Hongbing Lu and David A. Rubenstein. Journal of Biomedical Materials Research: Part A. 2010 March 15; 92(4): 1431-1439. (DOI: 10.1002/jbm.a.32476 ).
- Dose effect of shear stress on platelet complement activation in a cone and plate shearing device. Wei Yin and David A. Rubenstein. Cellular and Molecular Bioengineering, June 2009, Vol. 2, No. 2: 274-280. (DOI: 10.1007/s12195-009-0055-9).
- Glycated Albumin Modulates Platelet Susceptibility to Flow Induced Activation and Aggregation. David A. Rubenstein and Wei Yin. Platelets, May 2009; 20(3): 206-215. (DOI: 10.1080/09537100902795492).
- Bioassay chamber for angiogenesis with perfused explanted arteries and electrospun scaffolding. David Rubenstein, Dong Han, Sara Goldgraben, Hebah El-Gendi, Pelagia-Irene Gouma and Mary D. Frame. Microcirculation. 2007: 14, 723-737. (DOI: 10.1080/10739680701410173).
- Platelet factor V supports hemostasis in a patient with an acquired factor V inhibitor, as shown by prothrombinase and tenase assays. Maria T. Grosse Perdekamp, MD, David A. Rubenstein, Jolyon Jesty, PhD and Mae B. Hultin, MD. Blood Coagulation & Fibrinolysis 2006; Vol. 17, Issue October: 593-597. (DOI: 10.1097/01.mbc.0000245297.64644.ee).
- Activation of platelets exposed to shear stress in the presence of smoke extracts of low-nicotine and zero-nicotine cigarettes: the protective effect of nicotine. Jaimohan Ramachandran, David Rubenstein, Danny Bluestein and Jolyon Jesty. Nicotine and Tobacco Research. 2004; Vol. 6, No 5, Issue October: 835-841. (DOI: 10.1080/1462220042000274284).
- A shear-restricted pathway of platelet procoagulant activity is regulated by IQGAP1. Wadie Bahou, Lesley Scudder, David Rubenstein and Jolyon Jesty. Journal of Biological Chemistry. 2004; Vol. 279, No 21, Issue May 21: 22571-22577. (DOI: 10.1074/jbc.M402561200).
- Differences between mainstream and sidestream cigarette smoke extracts and nicotine in the activation of platelets under static and flow conditions. David Rubenstein, Jolyon Jesty and Danny Bluestein. Circulation. 2004; 109: 78-83. (DOI: 10.1161/01.CIR.0000108395.12766.25).
- National Institutes of Health
- American Heart Association
- National Science Foundation
- BME 260 - Statics and Dynamics in Biological Systems (SBU)
- BME 353 - Introduction to Biomaterials (SBU)
- BME 430 - Quantitative Human Physiology (SBU)
- BME 501 - Engineering Principles and Practices for Cell Biology (SBU)
- ENGR 1111 - Introduction to Engineering (OSU)
- ENSC 2123 - Elementary Dynamics (OSU)
- MAE 3013 - Mechanical and Aerospace Engineering Analysis (OSU)
- MAE 3233 - Heat Transfer (OSU)
- MAE 5003 - Advanced Biomaterials Science and Engineering (OSU)
- MAE 5023 - Advanced Biofluid Mechanics (OSU)